Clair de Lune, Debussy
another lab day!
today I extracted some DNA from our fli1a:eGFP background and1/2 mutants so that we can genotype them. I ran an RT-PCR and I’ll do the gel tomorrow to determine their genotype (all with the help of the amazing MSc. student i’m working under).
the fish are all currently really small so it was harder to cut their fins, but I think it went well for my first time! hopefully my PCR is successful too...
the fins regenerate in about a week, so these fish will be good as new soon!
The following suggestions may be helpful for a person who finds that they are struggling with low self-esteem:
1. It starts with a decision to be your own person. Don’t live your life to please, or impress, others.
2. Try to grasp the fact we have different goals and values … And don’t be swayed by people who criticise your dreams.
3. Don’t compare your journey to someone else’s journey as we start from different places and face different challenges.
4. Be kind, understanding and patient with yourself. Accept mistakes and failures are part of all our lives. Also, choose to frame mistakes as learning opportunities.
5. You need to root for yourself, and seek to be your own best friend. Don’t put yourself down – in public, or when you’re alone.
6. Remind yourself a weakness can become a strength in time. It takes patience and effort – but eventually things change.
7. Make a list of what you’re good at, and keep adding to the list. Also, note the strengths that others see, and comment on, as well.
8. Treat yourself with respect and praise the things that you do
9. Find ways to dissipate and channel negative emotions. Don’t allow them to dictate the way you start to see yourself.
10. Spend time with those who like you, and can see your worth and value. Ignore those who attack you, and would like to see you fail.
11. Choose to stand up for yourself, and value being more assertive. Also, set and then enforce appropriate, healthy boundaries.
12. Admit your mistakes - then learn to laugh at yourself. It helps remove the pressure and the stress of “being perfect”.
Frida Kahlo - self portrait with cropped hair
kaitsbookcase
if you have 5 minutes...
watch a TED talk
light a candle
paint your nails
put on a hair/face mask
splash your face with cold water
walk around your house
stretch
have a small snack
make a cup of tea/coffee
clear your desk
if you have 10-15 minutes...
watch a longer TED talk
watch a youtube video on studying/something educational
do some yoga
go for a quick run
have a shower
make a smoothie
power nap
workout
organise your desk
if you have 20-35 minutes...
watch a longer TED talk
watch one short episode of a tv programme
do a longer workout
go for a longer run
go for a walk
bake something
call a friend
if you have 40-60 minutes...
watch a documentary
watch one longer episode of a tv programme
go for an even longer walk/run
have a bath
have a one hour nap to help you remember things
sometimes i just want to pinch myself - I can’t believe this is where I live & that these are the kinds of coffee shop views I get to experience on a daily basis.
“Make your education valuable. Apply what you learnt. Refuse to take the back seat and watch things happen. Join the change and be part of the change.”
- Israelmore Ayivor
Apollo 11 Hasselblad image from film magazine 38/O - Lunar orbit, Trans-Earth coast. x
Tonight this woman returns from space, the all-time US record-holder. Commander Peggy Whitson PhD, you rock.
fly me to the moon, let me play among the stars, let me see what spring is like on jupiter and mars.
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