Another Lab Day!

Another Lab Day!
Another Lab Day!
Another Lab Day!

another lab day!

today I extracted some DNA from our fli1a:eGFP background and1/2 mutants so that we can genotype them. I ran an RT-PCR and I’ll do the gel tomorrow to determine their genotype (all with the help of the amazing MSc. student i’m working under).

the fish are all currently really small so it was harder to cut their fins, but I think it went well for my first time! hopefully my PCR is successful too...

the fins regenerate in about a week, so these fish will be good as new soon!

More Posts from Alyssanicoleguajardo-blog and Others

I'm taking A Gothic Literature Class And I’m Absolutely Obsessed 

i'm taking a gothic literature class and I’m absolutely obsessed 

Sometimes I Just Want To Pinch Myself - I Can’t Believe This Is Where I Live & That These Are The Kinds

sometimes i just want to pinch myself - I can’t believe this is where I live & that these are the kinds of coffee shop views I get to experience on a daily basis. 

From Beginner to Intermediate: an intense plan for advancing in language

Introduction

I've studied Spanish at school for 3 years and now I'm at a low B1 level. I can actually understand pretty well while listening or reading but I can't communicate fluently.

This plan will include vocabulary build up, some grammar revision, a lot of listening, reading and writing. And could be used for the most languages, not only Spanish.

Plan

Every day:

Conjugate one verb in present, past and future tenses

Make a list about 10 - 30 words long

Create flashcards with them and start learning them (I use Quizlet for flashcards)

Revise yesterday's set of flashcards

2-3 times a week:

Read an article or a few pages from a book

Write a few sentences about anything in your target language

Listen to one episode of podcast (at least one)

Once a week or every two weeks:

Watch a movie in your target language, preferably animated movie as the language used there is easier. You can watch with subtitles

Grammar exercises

Translate some short text

Once a month:

Write something longer, like an essay or report, on chosen topic

Additionally:

Talk to yourself, to your friends, to your pets

Text with someone

Look at the transcription while listening to the podcast for second time

Repeat what you hear (in podcast or movie)

Check words you don't know from the listening and reading

Read out loud

Listen to music in your target language - you can even learn the text and sing along

Watch YouTube in your target language

Change your phone language to the one you're learning

Think in you target language!!!

***This is very intense plan for self-learners, you don't have to do all of these things in the given time. Adjust it to your own pace. I'll try to stick to this, if I have enough time.***

Breakfast In Bed & Monday’s WSJ

breakfast in bed & monday’s WSJ

I Can’t Even Handle These 🥰🌈🏳️‍🌈
I Can’t Even Handle These 🥰🌈🏳️‍🌈
I Can’t Even Handle These 🥰🌈🏳️‍🌈
I Can’t Even Handle These 🥰🌈🏳️‍🌈
I Can’t Even Handle These 🥰🌈🏳️‍🌈

I can’t even handle these 🥰🌈🏳️‍🌈

@prism

‘nocturne op. 9 no. 2’ by chopin but you’re in a hotel lobby avoiding the rain on a cold night in an unfamiliar city, captivated by the soft piano music that reminds you of home. (youtube)

Power should act and not talk.

Johann Wolfgang von Goethe, Maxims and Reflections (via philosophybits)

Happy Earth Day!!!

Happy Earth Day!!!

"Ere many generations pass, our machinery will be driven by a power obtainable at any point of the universe. 

"This idea is not novel. Men have been led to it long ago by instinct or reason; it has been expressed in many ways, and in many places, in the history of old and new. We find it in the delightful myth of Antaeus, who derives power from the earth; we find it among the subtle speculations of one of your splendid mathematicians and in many hints and statements of thinkers of the present time. 

"Throughout space there is energy. Is this energy static or kinetic! If static our hopes are in vain; if kinetic — and this we know it is, for certain — then it is a mere question of time when men will succeed in attaching their machinery to the very wheelwork of nature.

–Nikola Tesla

"Experiments With Alternate Currents Of High Potential And High Frequency," February 1892.

Happy Earth Day!!!
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fly me to the moon, let me play among the stars, let me see what spring is like on jupiter and mars.

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